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Detection of Salmonella strains and Escherichia coli O157:H7 in feces of small ruminants and their isolation with various media.

Pao S, Patel D, Kalantari A, Tritschler JP, Wildeus S, Sayre BL

Virginia State University, Agricultural Research Station, P.O. Box 9061, Petersburg, VA 23806, USA. spao@vsu.edu

Salmonella strains and Escherichia coli O157:H7 were detected in 17 and 5 small ruminants in Virginia, respectively, of 287 tested. Background microflora interfered with the fecal analysis. The combination of Salmonella enzyme immunoassay (EIA) detection and xylose-lysine-deoxycholate agar isolation was satisfactory. Modifying enrichment to a 1:100 dilution enabled effective E. coli O157:H7 detection by EIA and isolation by sorbitol-MacConkey agar with cefixime-tellurite.

Published 6 April 2005 in Appl Environ Microbiol, 71(4): 2158-61.
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