Salmonella Research Today is a free monthly online journal that collates and summarizes the latest research about Salmonella, including details on salmonella typhimurium, food poisoning, infection, treatment.

An allele of gyrA prevents Salmonella enterica serovar Typhimurium from using succinate as a carbon source.

Schmitz GE, Downs DM

Department of Bacteriology, University of Wisconsin-Madison, 420 Henry Mall, Rm. 120, Madison, WI 53706, USA.

A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C(4)-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C(4)-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.

Published 4 April 2006 in J Bacteriol, 188(8): 3126-9.
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