Salmonella Research Today is a free monthly online journal that collates and summarizes the latest research about Salmonella, including details on salmonella typhimurium, food poisoning, infection, treatment.

Characterization of Salmonella bacteriophages isolated from swine lagoon effluent.

McLaughlin MR, King RA

United States Department of Agriculture, Agricultural Research Service, Waste Management and Forage Research Unit, 810 Highway 12 East, PO Box 5367, MS 39762, USA. mike.mclaughlin@ars.usda.gov

Four Salmonella bacteriophages that had been originally isolated from swine manure lagoons were characterized and compared to each other and to well-known Salmonella phages P22 and Felix 01. Host ranges of the lagoon phages were similar to each other in spot tests on reference strains of Salmonella, but differed slightly from each other on a panel of Salmonella lagoon strains. In single-step growth at 35 degrees C the lagoon phages had latent periods of 15 to 20 min and burst sizes from 100 to 230. The lagoon phages and P22 were purified by cesium chloride (CsCl) gradient centrifugation and used to produce specific antisera and DNA. The lagoon phages were indistinguishable from each other but distinct from P22 and Felix 01 in immunodiffusion and infectivity neutralization tests and in restriction digest analysis.

Published 7 February 2008 in Curr Microbiol, 56(3): 208-13.
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